All

What are you looking for?

All
Projects
Results
Organizations

Quick search

  • Projects supported by TA ČR
  • Excellent projects
  • Projects with the highest public support
  • Current projects

Smart search

  • That is how I find a specific +word
  • That is how I leave the -word out of the results
  • “That is how I can find the whole phrase”

Polysome Profile Analysis - Yeast

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10192470" target="_blank" >RIV/00216208:11310/13:10192470 - isvavai.cz</a>

  • Alternative codes found

    RIV/61388971:_____/13:00425692

  • Result on the web

    <a href="http://dx.doi.org/10.1016/B978-0-12-420037-1.00009-9" target="_blank" >http://dx.doi.org/10.1016/B978-0-12-420037-1.00009-9</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/B978-0-12-420037-1.00009-9" target="_blank" >10.1016/B978-0-12-420037-1.00009-9</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Polysome Profile Analysis - Yeast

  • Original language description

    More than one 80 S monosome can translate an mRNA molecule at a time producing polysomes. The most widely used method to separate 40 S and 60 S ribosomal subunits from 80 S monosomes and polysomes is a high-velocity centrifugation of whole cell extractsin linear sucrose gradients. This polysome profile analysis technique has been routinely used to monitor translational fitness of cells under a variety of physiological conditions, to investigate functions of initiation factors involved in translation, to reveal defects in ribosome biogenesis, to determine roles of 5' UTR structures on mRNA translatability, and more recently for examination of miRNA-mediated translational repression (see an application of this protocol on Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae).

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2013

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Methods in Enzymology

  • ISSN

    0076-6879

  • e-ISSN

  • Volume of the periodical

    530

  • Issue of the periodical within the volume

    2013

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    9

  • Pages from-to

    173-181

  • UT code for WoS article

    000326098000010

  • EID of the result in the Scopus database