Polysome Profile Analysis - Yeast
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10192470" target="_blank" >RIV/00216208:11310/13:10192470 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/13:00425692
Result on the web
<a href="http://dx.doi.org/10.1016/B978-0-12-420037-1.00009-9" target="_blank" >http://dx.doi.org/10.1016/B978-0-12-420037-1.00009-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/B978-0-12-420037-1.00009-9" target="_blank" >10.1016/B978-0-12-420037-1.00009-9</a>
Alternative languages
Result language
angličtina
Original language name
Polysome Profile Analysis - Yeast
Original language description
More than one 80 S monosome can translate an mRNA molecule at a time producing polysomes. The most widely used method to separate 40 S and 60 S ribosomal subunits from 80 S monosomes and polysomes is a high-velocity centrifugation of whole cell extractsin linear sucrose gradients. This polysome profile analysis technique has been routinely used to monitor translational fitness of cells under a variety of physiological conditions, to investigate functions of initiation factors involved in translation, to reveal defects in ribosome biogenesis, to determine roles of 5' UTR structures on mRNA translatability, and more recently for examination of miRNA-mediated translational repression (see an application of this protocol on Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae).
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Methods in Enzymology
ISSN
0076-6879
e-ISSN
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Volume of the periodical
530
Issue of the periodical within the volume
2013
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
173-181
UT code for WoS article
000326098000010
EID of the result in the Scopus database
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