The choice of reference gene set for assessing gene expression in barley (Hordeum vulgare L.) under low temperature and drought stress

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10195352" target="_blank" >RIV/00216208:11310/13:10195352 - isvavai.cz</a>

Alternative codes found

RIV/00027006:_____/13:00002634

Result on the web

<a href="http://dx.doi.org/10.1007/s00438-013-0774-4" target="_blank" >http://dx.doi.org/10.1007/s00438-013-0774-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00438-013-0774-4" target="_blank" >10.1007/s00438-013-0774-4</a>

Result language

angličtina

Original language name

The choice of reference gene set for assessing gene expression in barley (Hordeum vulgare L.) under low temperature and drought stress

Original language description

Drought and low temperature are the two most significant causes of abiotic stress in agricultural crops and, therefore, they pose considerable challenges in plant science. Hence, it is crucial to study response mechanisms and to select genes for identification signaling pathways that lead from stimulus to response. The assessment of gene expression is often attempted using real-time RT-PCR (qRT-PCR), a technique which requires a careful choice of reference gene(s) for normalization purpose. Here, we report a comparison of 13 potential reference genes for studying gene expression in the leaf and crown of barley seedlings subjected to low temperature or drought stress. All three currently available software packages designed to identify reference genes from qRT-PCR data (GeNorm, NormFinder and BestKeeper) were used to identify informative sets of up to three reference genes. Interestingly, the data obtained from the separate treatment of leaf and crown have led to the recommendations tha

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Genetics and Genomics

ISSN

1617-4615

e-ISSN

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Volume of the periodical

288

Issue of the periodical within the volume

11

Country of publishing house

DE - GERMANY

Number of pages

11

Pages from-to

639-649

UT code for WoS article

000326263900008

EID of the result in the Scopus database

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