Identification of the most suitable reference gene for gene expression studies with development and abiotic stress response in Bromus sterilis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F21%3A88334" target="_blank" >RIV/60460709:41210/21:88334 - isvavai.cz</a>
Alternative codes found
RIV/60460709:41320/21:88334
Result on the web
<a href="https://www.nature.com/articles/s41598-021-92780-1#Abs1" target="_blank" >https://www.nature.com/articles/s41598-021-92780-1#Abs1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-021-92780-1" target="_blank" >10.1038/s41598-021-92780-1</a>

Result language
angličtina
Original language name
Identification of the most suitable reference gene for gene expression studies with development and abiotic stress response in Bromus sterilis
Original language description
Bromus sterilis is an annual weedy grass, causing high yield losses in winter cereals. Frequent use of herbicides had led to the evolution of herbicide resistance in this species. Mechanisms underlying herbicide resistance in B. sterilis must be uncovered because this problem is becoming a global threat. qRT-PCR and the next-generation sequencing technologies can elucidate the resistance mechanisms. Although qRT-PCR can calculate precise fold changes, its preciseness depends on the expression of reference genes. Regardless of stable expression in any given condition, no gene can act as a universal reference gene. Hence, it is necessary to identify the suitable reference gene for each species. To our knowledge, there are no reports on the suitable reference gene in any brome species so far. Thus, in this paper, the stability of eight genes was evaluated using qRT-PCR experiments followed by expression stability ranking via five most commonly used software for reference gene selection. Our findings sug
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project
<a href="/en/project/QK1820081" target="_blank" >QK1820081: Resistance monitoring methods of economically important pests and weeds to pesticides and anti-resistance strategies</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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