Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10218613" target="_blank" >RIV/00216208:11310/14:10218613 - isvavai.cz</a>

Alternative codes found

RIV/86652036:_____/14:00441484 RIV/61388971:_____/14:00435141 RIV/61389013:_____/14:00435141

Result on the web

<a href="http://nar.oxfordjournals.org/content/42/8/5151.full.pdf+html" target="_blank" >http://nar.oxfordjournals.org/content/42/8/5151.full.pdf+html</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gku113" target="_blank" >10.1093/nar/gku113</a>

Result language

angličtina

Original language name

Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis

Original language description

Bacterial RNA polymerase (RNAP) is an essential multisubunit protein complex required for gene expression. Here, we characterize YvgS (HelD) from Bacillus subtilis, a novel binding partner of RNAP. We show that HelD interacts with RNAP-core between the secondary channel of RNAP and the alpha subunits. Importantly, we demonstrate that HelD stimulates transcription in an ATP-dependent manner by enhancing transcriptional cycling and elongation. We demonstrate that the stimulatory effect of HelD can be amplified by a small subunit of RNAP, delta. In vivo, HelD is not essential but it is required for timely adaptations of the cell to changing environment. In summary, this study establishes HelD as a valid component of the bacterial transcription machinery.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Volume of the periodical

42

Issue of the periodical within the volume

8

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

5151-5163

UT code for WoS article

000336092300042

EID of the result in the Scopus database

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