Bodipy-Labeled Nucleoside Triphosphates for Polymerase Synthesis of Fluorescent DNA

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10285992" target="_blank" >RIV/00216208:11310/14:10285992 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/14:00438211
Result on the web
<a href="http://dx.doi.org/10.1021/bc5003554" target="_blank" >http://dx.doi.org/10.1021/bc5003554</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/bc5003554" target="_blank" >10.1021/bc5003554</a>

Result language
angličtina
Original language name
Bodipy-Labeled Nucleoside Triphosphates for Polymerase Synthesis of Fluorescent DNA
Original language description
New fluorescent nucleosides and nucleoside triphosphate (dNTPs) analogs bearing the F-Bodipy fluorophore linked through a short, flexible nonconjugate tether were synthesized. The Bodipy-labeled dNTPs were substrates for several DNA polymerases which incorporated them into DNA in primer extension, nicking enzyme amplification reaction, and polymerase chain reaction. The fluorescence of F-Bodipy is not quenched upon incorporation in DNA and can be detected both in solutions and on gels.
Czech name
—
Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
—

Project
<a href="/en/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Center of novel approaches to bioanalysis and molecular diagnostics</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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