Cyanide hydratase from Aspergillus niger K10: Overproduction in Escherichia coli, purification, characterization and use in continuous cyanide degradation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10289017" target="_blank" >RIV/00216208:11310/14:10289017 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/14:00428318
Result on the web
<a href="http://dx.doi.org/10.1016/j.procbio.2013.12.008" target="_blank" >http://dx.doi.org/10.1016/j.procbio.2013.12.008</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.procbio.2013.12.008" target="_blank" >10.1016/j.procbio.2013.12.008</a>

Result language
angličtina
Original language name
Cyanide hydratase from Aspergillus niger K10: Overproduction in Escherichia coli, purification, characterization and use in continuous cyanide degradation
Original language description
A cyanide hydratase from Aspergillus niger K10 was expressed in Escherichia coli and purified. Apart from HCN, it transformed some nitriles, preferentially 2-cyanopyridine and fumaronitrile. V-max and K-m for HCN were ca. 6.8mmol min(-1) mg(-1) protein and 109mM, respectively. V-max for fumaronitrite and 2-cyanopyridine was two to three orders of magnitude lower than for HCN (ca. 18.8 and 10.3 mu.,mol min(-1) mg(-1), respectively) but K-m was also lower (ca. 14.7 and 3.7 mM, respectively). Both cyanidehydratase and nitrilase activities were abolished in truncated enzyme variants missing 18-34 C-terminal aa residues. The enzyme exhibited the highest activity at 45 degrees C and pH 8-9; it was unstable at over 35 degrees C and at below pH 5.5. The operational stability of the whole-cell catalyst was examined in continuous stirred membrane reactors with 70-mL working volume. The catalyst exhibited a half-life of 5.6 h at 28 degrees C. A reactor loaded with an excess of the catalyst was u
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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