Acute morphine affects the rat circadian clock via rhythms of phosphorylated ERK1/2 and GSK3 kinases and Per1 expression in the rat suprachiasmatic nucleus

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10297866" target="_blank" >RIV/00216208:11310/15:10297866 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/bph.13152" target="_blank" >http://dx.doi.org/10.1111/bph.13152</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/bph.13152" target="_blank" >10.1111/bph.13152</a>

Result language
angličtina
Original language name
Acute morphine affects the rat circadian clock via rhythms of phosphorylated ERK1/2 and GSK3 kinases and Per1 expression in the rat suprachiasmatic nucleus
Original language description
Background and PurposeOpioids affect the circadian clock and may change the timing of many physiological processes. This study was undertaken to investigate the daily changes in sensitivity of the circadian pacemaker to an analgesic dose of morphine, andto uncover a possible interplay between circadian and opioid signalling. Experimental ApproachA time-dependent effect of morphine (1mgkg(-1), i.p.) applied either during the day or during the early night was followed, and the levels of phosphorylated ERK1/2, GSK3, c-Fos and Per genes were assessed by immunohistochemistry and in situ hybridization. The effect of morphine pretreatment on light-induced pERK and c-Fos was examined, and day/night difference in activity of opioid receptors was evaluated by [S-35]-GTPS binding assay. Key ResultsMorphine stimulated a rise in pERK1/2 and pGSK3 levels in the suprachiasmatic nucleus (SCN) when applied during the day but significantly reduced both kinases when applied during the night. Morphine at
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FH - Neurology, neuro-surgery, nuero-sciences
OECD FORD branch
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Project
<a href="/en/project/GAP303%2F12%2F1137" target="_blank" >GAP303/12/1137: The role of the STAT signalling pathway in the rat circadian system</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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