Translation initiation factor elF3 promotes programmed stop codon readthrough

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10317650" target="_blank" >RIV/00216208:11310/15:10317650 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/15:00452640
Result on the web
<a href="http://dx.doi.org/10.1093/nar/gkv421" target="_blank" >http://dx.doi.org/10.1093/nar/gkv421</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/nar/gkv421" target="_blank" >10.1093/nar/gkv421</a>

Result language
angličtina
Original language name
Translation initiation factor elF3 promotes programmed stop codon readthrough
Original language description
Programmed stop codon readthrough is a post-transcription regulatory mechanism specifically increasing proteome diversity by creating a pool of C-terminally extended proteins. During this process, the stop codon is decoded as a sense codon by a near-cognate tRNA, which programs the ribosome to continue elongation. The efficiency of competition for the stop codon between release factors (eRFs) and near-cognate tRNAs is largely dependent on its nucleotide context; however, the molecular mechanism underlying this process is unknown. Here, we show that it is the translation initiation (not termination) factor, namely eIF3, which critically promotes programmed readthrough on all three stop codons. In order to do so, eIF3 must associate with pre-termination complexes where it interferes with the eRF1 decoding of the third/wobble position of the stop codon set in the unfavorable termination context, thus allowing incorporation of near-cognate tRNAs with a mismatch at the same position. We clearly demonstrate that efficient readthrough is enabled by near-cognate tRNAs with a mismatch only at the third/wobble position. Importantly, the eIF3 role in programmed readthrough is conserved between yeast and humans.
Czech name
—
Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—

Project
<a href="/en/project/GA14-05394S" target="_blank" >GA14-05394S: Structure and function of the human translation initiation factor 3 (eIF3) and its associated eIFs.</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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