Yeast applied readthrough inducing system (YARIS): an invivo assay for the comprehensive study of translational readthrough
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00518352" target="_blank" >RIV/61388971:_____/19:00518352 - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/nar/article/47/12/6339/5487265" target="_blank" >https://academic.oup.com/nar/article/47/12/6339/5487265</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/nar/gkz346" target="_blank" >10.1093/nar/gkz346</a>
Alternative languages
Result language
angličtina
Original language name
Yeast applied readthrough inducing system (YARIS): an invivo assay for the comprehensive study of translational readthrough
Original language description
Stop codon readthrough-the decoding of a stop codon by a near-cognate tRNA-is employed by viruses to balance levels of enzymatic and structural proteins and by eukaryotic cells to enable isoform-specific protein synthesis in response to external stimuli. Owing to the prevalence of premature termination codons in human disease, readthrough has emerged as an attractive therapeutic target. A growing list of various features, for example the +4 nucleotide immediately following the stop codon, modulate readthrough levels, underscoring the need for systematic investigation of readthrough. Here, we identified and described a complete group of yeast tRNAs that induce readthrough in the stop-codon tetranucleotide manner when overexpressed, designated readthrough-inducing tRNAs (rti-tRNAs). These rti-tRNAs are the keystones of YARIS (yeast applied readthrough inducing system), a reporter-based assay enabling simultaneous detection of readthrough levels at all twelve stop-codon tetranucleotides and as a function of the complete set of rti-tRNAs. We demonstrate the utility of YARIS for systematic study of translation readthrough by employing it to interrogate the effects of natural rti-tRNA modifications, as well as various readthrough-inducing drugs (RTIDs). This analysis identified a variety of genetic interactions demonstrating the power of YARIS to characterize existing and identify novel RTIDs. © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/GA18-02014S" target="_blank" >GA18-02014S: Neglected players in human non-sense readthrough coming to light.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nucleic Acids Research
ISSN
1362-4962
e-ISSN
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Volume of the periodical
47
Issue of the periodical within the volume
12
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
6339-6350
UT code for WoS article
000475891900030
EID of the result in the Scopus database
2-s2.0-85069293930