Rules of UGA-N decoding by near-cognate tRNAs and analysis of readthrough on short uORFs in yeast
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F16%3A00460004" target="_blank" >RIV/61388971:_____/16:00460004 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/16:10332396
Result on the web
<a href="http://dx.doi.org/10.1261/rna.054452.115" target="_blank" >http://dx.doi.org/10.1261/rna.054452.115</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1261/rna.054452.115" target="_blank" >10.1261/rna.054452.115</a>
Alternative languages
Result language
angličtina
Original language name
Rules of UGA-N decoding by near-cognate tRNAs and analysis of readthrough on short uORFs in yeast
Original language description
The molecular mechanism of stop codon recognition by the release factor eRF1 in complex with eRF3 has been described in great detail; however, our understanding of what determines the difference in termination efficiencies among various stop codon tetranucleotides and how near-cognate (nc) tRNAs recode stop codons during programmed readthrough in Saccharomyces cerevisiae is still poor. Here, we show that UGA-C as the only tetranucleotide of all four possible combinations dramatically exacerbated the readthrough phenotype of the stop codon recognition-deficient mutants in eRF1. Since the same is true also for UAA-C and UAG-C, we propose that the exceptionally high readthrough levels that all three stop codons display when followed by cytosine are partially caused by the compromised sampling ability of eRF1, which specifically senses cytosine at the +4 position.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
R N A
ISSN
1355-8382
e-ISSN
—
Volume of the periodical
22
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
456-466
UT code for WoS article
000371365400013
EID of the result in the Scopus database
2-s2.0-84958719751