Cysteine tRNA acts as a stop codon readthrough-inducing tRNA in the human HEK293T cell line
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F23%3A00576287" target="_blank" >RIV/61388971:_____/23:00576287 - isvavai.cz</a>
Result on the web
<a href="https://rnajournal.cshlp.org/content/29/9/1379" target="_blank" >https://rnajournal.cshlp.org/content/29/9/1379</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1261/rna.079688.123" target="_blank" >10.1261/rna.079688.123</a>
Alternative languages
Result language
angličtina
Original language name
Cysteine tRNA acts as a stop codon readthrough-inducing tRNA in the human HEK293T cell line
Original language description
Under certain circumstances, any of the three termination codons can be read through by a near-cognate tRNA, i.e., a tRNA whose two out of three anticodon nucleotides base pair with those of the stop codon. Unless programed to synthetize C-terminally extended protein variants with expanded physiological roles, readthrough represents an undesirable translational error. On the other side of a coin, a significant number of human genetic diseases is associated with the introduction of nonsense mutations (premature termination codons [PTCs]) into coding sequences, where stopping is not desirable. Here, the tRNA's ability to induce readthrough opens up the intriguing possibility of mitigating the deleterious effects of PTCs on human health. In yeast, the UGA and UAR stop codons were described to be read through by four readthrough-inducing rti-tRNAs-tRNA(Trp) and tRNA(Cys), and tRNA(Tyr) and tRNA(Gln), respectively. The readthrough-inducing potential of tRNA(Trp) and tRNA(Tyr) was also observed in human cell lines. Here, we investigated the readthrough-inducing potential of human tRNA(Cys) in the HEK293T cell line. The tRNA(Cys) family consists of two isoacceptors, one with ACA and the other with GCA anticodons. We selected nine representative tRNA(Cys) isodecoders (differing in primary sequence and expression level) and tested them using dual luciferase reporter assays. We found that at least two tRNA(Cys) can significantly elevate UGA readthrough when overexpressed. This indicates a mechanistically conserved nature of rti-tRNAs between yeast and human, supporting the idea that they could be used in the PTC-associated RNA therapies.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
RNA
ISSN
1355-8382
e-ISSN
1469-9001
Volume of the periodical
29
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
1379-1387
UT code for WoS article
001049266600001
EID of the result in the Scopus database
2-s2.0-85168252001