Interactions of of helquats with chiral acidic aromatic analytes investigated by partial-filling affinity capillary electrophoresis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F16%3A10329365" target="_blank" >RIV/00216208:11310/16:10329365 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.chroma.2016.08.053" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2016.08.053</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2016.08.0532-s2.0-84991054850" target="_blank" >10.1016/j.chroma.2016.08.0532-s2.0-84991054850</a>

Result language

angličtina

Original language name

Interactions of of helquats with chiral acidic aromatic analytes investigated by partial-filling affinity capillary electrophoresis

Original language description

Noncovalent molecular interactions between helquats, a new class of dicationic helical extended diquats, and several chiral acidic aromatic drugs and catalysts have been investigated using partial-filling affinity capillary electrophoresis (PF-ACE). Helquats dissolved at 1 mM concentration in the aqueous background electrolyte (40 mM Tris, 20 mM acetic acid, pH 8.1) were introduced as ligand zones of variable length (0-130 mm) into the hydroxypropylcellulose coated fused silica capillary whereas 0.1 mM solutions of negatively charged chiral drugs or catalysts (warfarin, ibuprofen, mandelic acid, etodolac, binaphthyl phosphate and 11 other acidic aromatic compounds) were applied as a short analyte zone at the injection capillary end. After application of electric field, analyte and ligand migrated against each other and in case of their interactions, migration time of the analyte was increasing with increasing length of the ligand zone. From the tested compounds, only isomers of those exhibiting helical chirality and/or possessing conjugated aromatic systems were enantioselectively separated through their differential interactions with helquats. Some compounds with conjugated aromatic groups interacted with helquats moderately strongly but non-enantiospecifically. Small compounds with single benzene ring exhibited no or very weak non-enantiospecific interactions. PF-ACE method allowed to determine binding constants of the analyte-helquat complexes from the changes of migration times of the analytes. Binding constants of the weakest complexes of the analytes with helquats were less than 50 L/mol, whereas binding constants of the strongest complexes were in the range 1 000-1 400 L/mol.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

—

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Volume of the periodical

1467

Issue of the periodical within the volume

7 October 2016

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

10

Pages from-to

417-426

UT code for WoS article

000385323800040

EID of the result in the Scopus database

2-s2.0-84991054850