Determination of binding constants of human insulin complexes with serotonin, dopamine, arginine, and phenol by pressure assisted partial filling affinity capillary electrophoresis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F18%3A00498027" target="_blank" >RIV/61388963:_____/18:00498027 - isvavai.cz</a>
Result on the web
<a href="http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf" target="_blank" >http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Determination of binding constants of human insulin complexes with serotonin, dopamine, arginine, and phenol by pressure assisted partial filling affinity capillary electrophoresis
Original language description
A new method, pressure assisted partial filling affinity capillary electrophoresis (PF-ACE), has been developed to study noncovalent interactions of the hexamer of human insulin (HI) with cationic ligands, such as phenolic neurotransmitters serotonin and dopamine, and amino acid arginine, or with anionic ligand phenol, in alkaline aqueous solutions. The apparent binding constants, Kb, of the HI-ligand complexes were determined from the dependence of the effective migration time changes of the above ligands on the variable zone lengths of HI dissolved in the background electrolyte and hydrodynamically introduced into the bare fused silica capillary close to the UV detector. The HI interactions with the above ligands were found to be moderately strong, with Kb values in the range 385-1314 L/mol.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis
ISBN
978-80-904959-5-1
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
272-275
Publisher name
Ústav analytické chemie AV ČR, v. v. i.
Place of publication
Brno
Event location
Brno
Event date
Oct 15, 2018
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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