Study of deoxyribonucleic acid-ligand interactions by partial filling affinity capillary electrophoresis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F14%3A00430010" target="_blank" >RIV/61388963:_____/14:00430010 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/14:10323802
Result on the web
<a href="http://dx.doi.org/10.1016/j.chroma.2014.04.061" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2014.04.061</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2014.04.061" target="_blank" >10.1016/j.chroma.2014.04.061</a>

Result language
angličtina
Original language name
Study of deoxyribonucleic acid-ligand interactions by partial filling affinity capillary electrophoresis
Original language description
In this work, a new partial filling affinity capillary electrophoresis (PF-ACE) method has been developed and applied to investigation of non-covalent molecular interactions between double stranded DNA oligonucleotide (Dickerson dodecamer) and classicalDNA intercalator ligand-ethidiumbromide (EtBr) or oligophenylene derivatives-based potential new type of DNA ligands. Binding constants of DNA-ligand complexes were determined from the dependence of migration time changes of DNA oligomer (applied as analyte) on the length of ligand zones introduced beforehand as plugs of various lengths (0-75 mm with 12.5 mm step) in hydroxypropylcellulose coated fused silica capillary of 50/375 mu m I.D./O.D. and 400/300 mm total/effective length. PF-ACE experiments were performed in two background electrolytes, Tris-borate, pH 8.0, ionic strength 14.3 mM (BGE1), and sodium phosphate, pH 7.5, ionic strength 133 mM (BGE2). Binding constants of DNA-EtBr complex (ca 15300 L/mol in the BGE1 and 4200 L/mol
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)