Spotsizer: High-throughput quantitative analysis of microbial growth
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F16%3A10329654" target="_blank" >RIV/00216208:11310/16:10329654 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.2144/000114459" target="_blank" >http://dx.doi.org/10.2144/000114459</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2144/000114459" target="_blank" >10.2144/000114459</a>
Alternative languages
Result language
angličtina
Original language name
Spotsizer: High-throughput quantitative analysis of microbial growth
Original language description
Microbial colony growth can serve as a useful readout in assays for studying complex genetic interactions or the effects of chemical compounds. Although computational tools for acquiring quantitative measurements of microbial colonies have been developed, their utility can be compromised by inflexible input image requirements, non-trivial installation procedures, or complicated operation. Here, we present the Spotsizer software tool for automated colony size measurements in images of robotically arrayed microbial colonies. Spotsizer features a convenient graphical user interface (GUI), has both single-image and batch-processing capabilities, and works with multiple input image formats and different colony grid types. We demonstrate how Spotsizer can be used for high-throughput quantitative analysis of fission yeast growth. The user-friendly Spotsizer tool provides rapid, accurate, and robust quantitative analyses of microbial growth in a high-throughput format. Spotsizer is freely available at https://data.csiro.au/dap/landingpage?pid=csiro:15330 under a proprietary CSIRO license.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GPP305%2F12%2FP040" target="_blank" >GPP305/12/P040: Fission yeast CSL proteins in the maintenance of genome integrity</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
BioTechniques
ISSN
0736-6205
e-ISSN
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Volume of the periodical
61
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
191-201
UT code for WoS article
000386195800006
EID of the result in the Scopus database
2-s2.0-84991018119