The effect of buffer concentration and cation type in the mobile phase on retention of amino acids and dipeptides in hydrophilic interaction liquid chromatography
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10373245" target="_blank" >RIV/00216208:11310/18:10373245 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1007/s11696-017-0265-x" target="_blank" >https://doi.org/10.1007/s11696-017-0265-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11696-017-0265-x" target="_blank" >10.1007/s11696-017-0265-x</a>
Alternative languages
Result language
angličtina
Original language name
The effect of buffer concentration and cation type in the mobile phase on retention of amino acids and dipeptides in hydrophilic interaction liquid chromatography
Original language description
The current work is focused on exploring the effect of buffer cation type and its concentration on retention of amino acids, dipeptides and their blocked analogues on two stationary phases, i.e., bare silica and amide-based in hydrophilic interaction liquid chromatography. Five different buffers of pH 4.0 composed of Tris/acetic acid, triethylamine/acetic acid, ammonium/acetic acid, Li+/acetic acid and Ba2+/acetic acid were used in various concentrations. Interestingly, an increase of the buffer concentration caused increasing, decreasing or stable retention of analytes, according to the cation type in the buffer. The buffers containing barium cations provided the highest retention of all the analytes in comparable mobile phases, i.e., buffers with the same ionic strength and pH on both columns. Moreover, using buffers with barium cation different selectivity for dipeptides was observed. The chromatographic systems with buffers consisting of triethylamine behaved differently compared to others.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA16-05942S" target="_blank" >GA16-05942S: Gradient chromatofocusing and isoelectric focusing - tools for separation of proteins</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chemical Papers
ISSN
0366-6352
e-ISSN
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Volume of the periodical
72
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
139-147
UT code for WoS article
000419790000014
EID of the result in the Scopus database
2-s2.0-85040368233