Study of the retention behavior of small polar molecules on different types of stationary phases used in hydrophilic interaction liquid chromatography

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F14%3A10282192" target="_blank" >RIV/00216208:11160/14:10282192 - isvavai.cz</a>

Result on the web

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201400020/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201400020/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201400020" target="_blank" >10.1002/jssc.201400020</a>

Result language

angličtina

Original language name

Study of the retention behavior of small polar molecules on different types of stationary phases used in hydrophilic interaction liquid chromatography

Original language description

The retention behavior of a large group of analytes (35) with varied properties (pK(a) and log P) was studied on eight hydrophilic interaction LC columns with different surfaces, stationary phase chemistries, and types of particles. The acetonitrile content (5-95%), buffer concentration (0.5-200 mM), and pH of the mobile phase (3.8 and 6.8) were evaluated for their effects on the retention behavior. The type of stationary phase had a significant impact on the selectivity and retention time of the testedanalytes. Completely different selectivity was observed on the aminopropyl stationary phase. In this study, the influence of the buffer concentration was similar for all tested columns, except for the aminopropyl stationary phase. Increasing the bufferconcentration led to decreased retention times for the basic compounds and increased retention times for the acidic compounds, while the inverse behavior was observed on the aminopropyl stationary phase. The selectivity of the individual

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/EE2.3.30.0022" target="_blank" >EE2.3.30.0022: Support of establishment, development, and mobility of quality research teams at the Charles University</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Volume of the periodical

37

Issue of the periodical within the volume

11

Country of publishing house

DE - GERMANY

Number of pages

11

Pages from-to

1297-1307

UT code for WoS article

000337679600011

EID of the result in the Scopus database

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