Amperometric Determination of Catecholamines by Enzymatic Biosensors in Flow Systems
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10376275" target="_blank" >RIV/00216208:11310/18:10376275 - isvavai.cz</a>
Alternative codes found
RIV/61388955:_____/18:00490697
Result on the web
<a href="https://doi.org/10.1002/elan.201800078" target="_blank" >https://doi.org/10.1002/elan.201800078</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elan.201800078" target="_blank" >10.1002/elan.201800078</a>
Alternative languages
Result language
angličtina
Original language name
Amperometric Determination of Catecholamines by Enzymatic Biosensors in Flow Systems
Original language description
Four various biosensors based on flow enzymatic mini-reactors containing one of the two different mesoporous silica powders (SBA-15 or MCM-41) modified by one of the two enzymes (laccase or tyrosinase) were used for the determination of L-DOPA, dopamine, noradrenaline, and adrenaline. Dependences of peak currents on detection potentials, flow rates, injected volumes, reactor volumes, and analyte concentrations were studied and optimized. The silica powder of MCM-41 with covalently bonded laccase (Lac) was found to be the best filling for the detection of catecholamines. Relative current response of such biosensor was: 100% for dopamine, 32.1% for L-DOPA, 26.2% for noradrenaline, and 0.71% for adrenaline. Statistical evaluation of parallel determinations of studied analytes with this biosensor proved its good accuracy (RSD=1.82-5.18%) and sensitivity (5.46-15.5molL(-1)). Life time of the studied biosensors is several months. The practical applicability of Lac-MCM41 biosensor was successfully verified by the determination of dopamine and noradrenaline in medical solutions for infusions.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electroanalysis
ISSN
1040-0397
e-ISSN
—
Volume of the periodical
30
Issue of the periodical within the volume
6
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
1163-1171
UT code for WoS article
000435271000026
EID of the result in the Scopus database
2-s2.0-85043703846