Fibroblasts potentiate melanoma cells in vitro invasiveness induced by UV-irradiated keratinocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10376833" target="_blank" >RIV/00216208:11310/18:10376833 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/18:00502203 RIV/00216208:11110/18:10376833

Result on the web

<a href="https://doi.org/10.1007/s00418-018-1650-4" target="_blank" >https://doi.org/10.1007/s00418-018-1650-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00418-018-1650-4" target="_blank" >10.1007/s00418-018-1650-4</a>

Result language

angličtina

Original language name

Fibroblasts potentiate melanoma cells in vitro invasiveness induced by UV-irradiated keratinocytes

Original language description

Melanoma represents a malignant disease with steadily increasing incidence. UV-irradiation is a recognized key factor in melanoma initiation. Therefore, the efficient prevention of UV tissue damage bears a critical potential for melanoma prevention. In this study, we tested the effect of UV irradiation of normal keratinocytes and their consequent interaction with normal and cancer-associated fibroblasts isolated from melanoma, respectively. Using this model of UV influenced microenvironment, we measured melanoma cell migration in 3-D collagen gels. These interactions were studied using DNA microarray technology, immunofluorescence staining, single cell electrophoresis assay, viability (dead/life) cell detection methods, and migration analysis. We observed that three 10 mJ/cm(2) fractions at equal intervals over 72 h applied on keratinocytes lead to a 50% increase (p &lt; 0.05) in in vitro invasion of melanoma cells. The introduction cancer-associated fibroblasts to such model further significantly stimulated melanoma cells in vitro invasiveness to a higher extent than normal fibroblasts. A panel of candidate gene products responsible for facilitation of melanoma cells invasion was defined with emphasis on IL-6, IL-8, and CXCL-1. In conclusion, this study demonstrates a synergistic effect between cancer microenvironment and UV irradiation in melanoma invasiveness under in vitro condition.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30106 - Anatomy and morphology (plant science to be 1.6)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Histochemistry and Cell Biology

ISSN

0948-6143

e-ISSN

—

Volume of the periodical

149

Issue of the periodical within the volume

5

Country of publishing house

DE - GERMANY

Number of pages

14

Pages from-to

503-516

UT code for WoS article

000433026100006

EID of the result in the Scopus database

2-s2.0-85041895906