Flow amperometric biosensor based on two enzymatic reactors (acetylcholinesterasecholine oxidase) for the detection of neurotransmitter acetylcholine

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F19%3A10409590" target="_blank" >RIV/00216208:11310/19:10409590 - isvavai.cz</a>
Result on the web
<a href="http://web.natur.cuni.cz/analchem/isc/isc15.pdf" target="_blank" >http://web.natur.cuni.cz/analchem/isc/isc15.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Flow amperometric biosensor based on two enzymatic reactors (acetylcholinesterasecholine oxidase) for the detection of neurotransmitter acetylcholine
Original language description
A stable amperometric biosensor with separated bienzymatic (acetylcholinesterase (AChE)-choline oxidase (ChOx)) part has been successfully developed for the determination of acetylcholine (ACh) in FIA for the first time. The bienzymatic part consists of two consecutively connected enzymatic reactors, which were independently prepared by covalent immobilization of AChE or ChOx on mesoporous silica powder (SBA-15). The amperometric measurements are based on the detection of consumed oxygen. The wall-jet cell with working silver solid amalgam electrode covered by mercury film to carry out four-electron oxygen reduction at the highly negative potential was used. The experimental parameters such as amounts of immobilized enzymes, pH of carrier solution, detection potential, flow rate, and injection volume of ACh were optimized. The detection limit was found to be 4.1 μmol L-1. The developed amperometric ACh biosensor showed good repeatability and long-term stability. The same enzymatic reactors were successfully used for 500 measurements during 90 days.
Czech name
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Czech description
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Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Proceedings of the 15th International Students Conference "Modern Analytical Chemistry"
ISBN
978-80-7444-068-7
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
61-66
Publisher name
Univerzita Karlova, Přírodovědecká fakulta
Place of publication
Praha
Event location
Prague
Event date
Sep 19, 2019
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000576787700010

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