Construction and Application of Flow Amperometric Biosensor Based on Enzymatic Reactor for Determination of Choline
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F19%3A00505279" target="_blank" >RIV/61388955:_____/19:00505279 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/19:10409215
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Construction and Application of Flow Amperometric Biosensor Based on Enzymatic Reactor for Determination of Choline
Original language description
In this contribution, a novel fast, simple and stable biosensor with the enzymatic reactor based on choline oxidase (ChOx) was developed and applied for the determination of choline using flow injection analysis with amperometric detection. The preparation of the reactor is based on the covalent immobilization of ChOx with glutaraldehyde to mesoporous silica powder (SBA-15) previously covered by NH2-groups. The experimental parameters affecting the sensitivity and stability of the biosensor were optimized. The proposed biosensor with the newly developed ChOx-based reactor possesses good repeatability, reproducibility, long-term stability, and reusability. It was successfully applied for the determination of choline in commercial pharmaceuticals.
Czech name
—
Czech description
—
Classification
Type
D - Article in proceedings
CEP classification
—
OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Result continuities
Project
<a href="/en/project/GA17-05387S" target="_blank" >GA17-05387S: New Approaches for Monitoring of Transport of Selected Xenobiotics across Biomimetic Membranes</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
XXXIX. Moderní elektrochemické metody. Sborník přednášek
ISBN
978-80-905221-7-6
ISSN
—
e-ISSN
—
Number of pages
5
Pages from-to
218-222
Publisher name
Best Servis
Place of publication
Ústí nad Labem
Event location
Jetřichovice
Event date
May 20, 2019
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000583689900051