Amperometric Biosensor Based on Enzymatic Reactor for Choline Determination in Flow Systems
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F19%3A00510097" target="_blank" >RIV/61388955:_____/19:00510097 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/19:10402080
Result on the web
<a href="http://hdl.handle.net/11104/0300653" target="_blank" >http://hdl.handle.net/11104/0300653</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elan.201900237" target="_blank" >10.1002/elan.201900237</a>
Alternative languages
Result language
angličtina
Original language name
Amperometric Biosensor Based on Enzymatic Reactor for Choline Determination in Flow Systems
Original language description
A simple, selective and stable biosensor with the enzymatic reactor based on choline oxidase (ChOx) was developed and applied for the determination of choline (Ch) in flow injection analysis with amperometric detection. The enzyme ChOx was covalently immobilized with glutaraldehyde to mesoporous silica powder (SBA-15) previously covered by NH2-groups. This powder was found as an optimal filling of the reactor. The detection of Ch is based on amperometric monitoring of consumed oxygen during the enzymatic reaction, which is directly proportional to Ch concentration. Two arrangements of an electrolytic cell in FIA, namely wall-jet cell with working silver solid amalgam electrode covered by mercury film and flow-through cell with tubular detector of polished silver solid amalgam were compared. The experimental parameters affecting the sensitivity and stability of the biosensor (i. e. pH of the carrier solution, volume of reactor, amount of the immobilized enzyme, the detection potential, flow rate, etc.) were optimized. Under the optimized conditions, the limit of detection was found to be 9.0×10−6 mol L−1. The Michaelis-Menten constant for covalently immobilized ChOx on SBA-15 was calculated. The proposed amperometric biosensor with the developed ChOx-based reactor exhibits good repeatability, reproducibility, long-term stability, and reusability. Its efficiency has been confirmed by the successful application for the determination of Ch in two commercial pharmaceuticals.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
<a href="/en/project/GA17-05387S" target="_blank" >GA17-05387S: New Approaches for Monitoring of Transport of Selected Xenobiotics across Biomimetic Membranes</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electroanalysis
ISSN
1040-0397
e-ISSN
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Volume of the periodical
31
Issue of the periodical within the volume
10
Country of publishing house
DE - GERMANY
Number of pages
12
Pages from-to
1901-1912
UT code for WoS article
000491476600009
EID of the result in the Scopus database
2-s2.0-85067473082