The efficiency of insulin production and its content in insulin-expressing model beta-cells correlate with their Zn(2+) levels

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10422305" target="_blank" >RIV/00216208:11310/20:10422305 - isvavai.cz</a>

Alternative codes found

RIV/00023001:_____/20:00080335

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=03XxDMikvV" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=03XxDMikvV</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1098/rsob.200137" target="_blank" >10.1098/rsob.200137</a>

Result language

angličtina

Original language name

The efficiency of insulin production and its content in insulin-expressing model beta-cells correlate with their Zn(2+) levels

Original language description

Insulin is produced and stored inside the pancreatic β-cell secretory granules, where it is assumed to form Zn(2+)-stabilized oligomers. However, the actual storage forms of this hormone and the impact of zinc ions on insulin production in vivo are not known. Our initial X-ray fluorescence experiment on granules from native Langerhans islets and insulinoma-derived INS-1E cells revealed a considerable difference in the zinc content. This led our further investigation to evaluate the impact of the intra-granular Zn(2+) levels on the production and storage of insulin in different model β-cells. Here, we systematically compared zinc and insulin contents in the permanent INS-1E and BRIN-BD11 β-cells and in the native rat pancreatic islets by flow cytometry, confocal microscopy, immunoblotting, specific messenger RNA (mRNA) and total insulin analysis. These studies revealed an impaired insulin production in the permanent β-cell lines with the diminished intracellular zinc content. The drop in insulin and Zn(2+) levels was paralleled by a lower expression of ZnT8 zinc transporter mRNA and hampered proinsulin processing/folding in both permanent cell lines. To summarize, we showed that the disruption of zinc homeostasis in the model β-cells correlated with their impaired insulin and ZnT8 production. This indicates a need for in-depth fundamental research about the role of zinc in insulin production and storage.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/NV16-28249A" target="_blank" >NV16-28249A: Pancreatic islets transplantation into the subcutaneous pouch - a preclinical study</a><br>

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Open Biology

ISSN

2046-2441

e-ISSN

—

Volume of the periodical

10

Issue of the periodical within the volume

10

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

200137

UT code for WoS article

000586600500002

EID of the result in the Scopus database

2-s2.0-85094221278