Clustering of the zeta-Chain Can Initiate T Cell Receptor Signaling
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10426295" target="_blank" >RIV/00216208:11310/20:10426295 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=xJYlJevQa6" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=xJYlJevQa6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms21103498" target="_blank" >10.3390/ijms21103498</a>
Alternative languages
Result language
angličtina
Original language name
Clustering of the zeta-Chain Can Initiate T Cell Receptor Signaling
Original language description
T cell activation is initiated when ligand binding to the T cell receptor (TCR) triggers intracellular phosphorylation of the TCR-CD3 complex. However, it remains unknown how biophysical properties of TCR engagement result in biochemical phosphorylation events. Here, we constructed an optogenetic tool that induces spatial clustering of zeta -chain in a light controlled manner. We showed that spatial clustering of the zeta -chain intracellular tail alone was sufficient to initialize T cell triggering including phosphorylation of zeta -chain, Zap70, PLC gamma, ERK and initiated Ca2+ flux. In reconstituted COS-7 cells, only Lck expression was required to initiate zeta -chain phosphorylation upon zeta -chain clustering, which leads to the recruitment of tandem SH2 domain of Zap70 from cell cytosol to the newly formed zeta -chain clusters at the plasma membrane. Taken together, our data demonstrated the biophysical relevance of receptor clustering in TCR signaling.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences [online]
ISSN
1422-0067
e-ISSN
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Volume of the periodical
21
Issue of the periodical within the volume
10
Country of publishing house
CH - SWITZERLAND
Number of pages
25
Pages from-to
3498
UT code for WoS article
000539312100094
EID of the result in the Scopus database
2-s2.0-85084963728