Analysis of Mitochondrial Retrograde Signaling in Yeast Model Systems
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F21%3A10428652" target="_blank" >RIV/00216208:11310/21:10428652 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/protocol/10.1007%2F978-1-0716-1266-8_6#citeas" target="_blank" >https://link.springer.com/protocol/10.1007%2F978-1-0716-1266-8_6#citeas</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-1266-8_6" target="_blank" >10.1007/978-1-0716-1266-8_6</a>
Alternative languages
Result language
angličtina
Original language name
Analysis of Mitochondrial Retrograde Signaling in Yeast Model Systems
Original language description
Mitochondrial retrograde signaling is a mitochondria-to-nucleus communication pathway, conserved from yeast to humans, by which dysfunctional mitochondria relay signals that lead to cell stress adaptation in physiopathological conditions via changes in nuclear gene expression. The most comprehensive picture of components and regulation of retrograde signaling has been obtained in Saccharomyces cerevisiae, where retrograde-target gene expression is regulated by RTG genes. In this chapter, we describe methods to measure mitochondrial retrograde pathway activation at the level of mRNA and protein products in yeast model systems, including cell suspensions and microcolonies. In particular, we will focus on three major procedures: mRNA levels of RTG-target genes, such as those encoding for peroxisomal citrate synthase (CIT2), aconitase, and NAD+-specific isocitrate dehydrogenase subunit 1 by real-time PCR; expression analysis of CIT2-gene protein product (Cit2p-GFP) by Western blot and fluorescence microscopy; the phosphorylation status of transcriptional factor Rtg1/3p which controls RTG-target gene transcription.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Mitochondrial Medicine, Volume 2: Assessing Mitochondria
ISBN
978-1-07-161265-1
Number of pages of the result
16
Pages from-to
87-102
Number of pages of the book
459
Publisher name
Springer
Place of publication
New York
UT code for WoS chapter
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