Production of Virus-Free Garlic Plants through Somatic Embryogenesis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F21%3A10432700" target="_blank" >RIV/00216208:11310/21:10432700 - isvavai.cz</a>

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=5By_SSfUzG" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=5By_SSfUzG</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/agronomy11050876" target="_blank" >10.3390/agronomy11050876</a>

Result language

angličtina

Original language name

Production of Virus-Free Garlic Plants through Somatic Embryogenesis

Original language description

The present study was conducted to establish a protocol for the regeneration of virus-free garlic plants through somatic embryogenesis of two Croatian garlic ecotypes. Basal parts of cloves from mother plants were cultured on a full Murashige and Skoog (MS) or modified MS medium (1/4 of KNO3 and NH4NO3 and 2xMgSO(4)) containing 0.1 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) or 1 mg L-1 2,4-D + 0.5 mg L-1 kinetin (Kin) and representing four different treatments. Plants were regenerated in MS medium containing 0.1 mg L-1 2,4-D and rooted in a medium containing 0.05 mg L-1 1-naphthaleneacetic acid (NAA) + 0.005 mg L-1 6-(gamma,gamma-dimethylallylamino)purine (2iP). The presence of viruses (i.e., sanitary status) of the mother plants and regenerants was checked by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). The mother plants were infected with onion yellow dwarf virus (OYDV) and leek yellow stripe virus (LYSV). In addition, the presence of garlic common latent virus (GCLV) was confirmed in four mother plants. Embryogenic callus developed in all four treatments with success ranging from 55% to 81% depending on treatment and ecotype. Plant conversion was significantly higher in somatic embryos developed in media containing 0.1 mg L-1 2,4-D than those developed in media containing 1 mg L-1 2,4-D + 0.5 mg L-1 Kin. Virus elimination success ranged from 13.3% up to 62.5% depending on garlic ecotype and treatment. The overall rate of virus elimination by somatic embryogenesis for both treatments and ecotypes were 20.7%, 22.9%, and 30.5% for OYDV, GCLV, and LYSV, respectively. Based on these results, somatic embryogenesis has been shown to be equally or more successful in eliminating garlic viruses compared to other in vitro methods.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Agronomy [online]

ISSN

2073-4395

e-ISSN

—

Volume of the periodical

11

Issue of the periodical within the volume

5

Country of publishing house

CH - SWITZERLAND

Number of pages

13

Pages from-to

876

UT code for WoS article

000653305200001

EID of the result in the Scopus database

2-s2.0-85106478468