Cytidine nucleoside analog is an effective antiviral drug against Trichomonasvirus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10444302" target="_blank" >RIV/00216208:11310/22:10444302 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=ZU6ZQmnYI9" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=ZU6ZQmnYI9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jmii.2021.08.008" target="_blank" >10.1016/j.jmii.2021.08.008</a>
Alternative languages
Result language
angličtina
Original language name
Cytidine nucleoside analog is an effective antiviral drug against Trichomonasvirus
Original language description
Background: Trichomonas vaginalis is the causative agent of a sexually transmitted disease in humans. The virulence of the parasite depends on multiple factors, including the presence of endosymbiotic dsRNA viruses. The presence of Trichomonasviruses (TVV) was associated with more severe genital symptoms, increased proinflammatory host reactions, and modulated parasite sensitivity to metronidazole. However, no efficient antiviral drugs are available against TVV to derive isogenic TVV-positive and TVV-negative cell lines that are essential for investigations of the TVV impact on T. vaginalis biology.Methods: 7-Deaza-20-C-methyladenosine (7d2CMA) and 20-C-methylcytidine (2CMC) were used for TVV inhibitory assay. TVV replication was monitored using quantitative reverse transcription PCR (RT qPCR) and western blotting. Modeling of TVV1 RNA-dependent RNA polymerase (RdRp) was performed to visualize the inhibitor-RdRp interaction. Susceptibility to metronidazole was performed under aerobic and anaerobic conditions.Results: We demonstrated that 2CMC but not 7d2CMA is a potent inhibitor of TVV replication. Molecular modeling suggested that the RdRp active site can accommodate 2CMC in the active triphosphate nucleotide form. The effect of 2CMC was shown on strains infected with a single and multiple TVV species. The optimal 2CMC concentration (10mM) demonstrated strong selectivity for TVVs over trichomonad growth. The presence of TVV has no effect on T. vaginalis metronidazole susceptibility in derived isogenic cell lines.Conclusions: 2CMC acts against TVVs and represents a new inhibitor against Totiviridae viruses. Our isogenic clones are now available for further studies of various aspects of T. vaginalis biology related to TVV infection. Copyright (c) 2021, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
<a href="/en/project/GC19-18773J" target="_blank" >GC19-18773J: Modulation of exosomal cargo by T. vaginalis endosymbionts</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Microbiology, Immunology and Infection
ISSN
1684-1182
e-ISSN
1995-9133
Volume of the periodical
55
Issue of the periodical within the volume
2
Country of publishing house
TW - TAIWAN (PROVINCE OF CHINA)
Number of pages
8
Pages from-to
191-198
UT code for WoS article
000796189400006
EID of the result in the Scopus database
2-s2.0-85114114433