A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10479986" target="_blank" >RIV/00216208:11310/22:10479986 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/22:00128587 RIV/68378050:_____/22:00568289
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=VWvNvaheV8" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=VWvNvaheV8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.xpro.2022.101199" target="_blank" >10.1016/j.xpro.2022.101199</a>
Alternative languages
Result language
angličtina
Original language name
A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines
Original language description
Primary cilia are hair-like sensory organelles protruding from the surface of most human cells. As cilia are dynamic, several aspects of their biology can only be revealed by real-time analysis in living cells. Here we describe the generation of primary cilia reporter cell lines. Furthermore, we provide a detailed protocol of how to use the reporter cell lines for live-cell imaging microscopy analysis of primary cilia to study their growth as well as intraciliary transport. For complete details on the use and execution of this protocol, please refer to Bernatik et al. (2020) and Pejskova et al. (2020).
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10600 - Biological sciences
Result continuities
Project
<a href="/en/project/LM2018129" target="_blank" >LM2018129: National Infrastructure for Biological and Medical Imaging Czech-BioImaging</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
STAR Protocols
ISSN
2666-1667
e-ISSN
2666-1667
Volume of the periodical
3
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
33
Pages from-to
101199
UT code for WoS article
001141790100017
EID of the result in the Scopus database
2-s2.0-85125437499