A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F22%3A00568289" target="_blank" >RIV/68378050:_____/22:00568289 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/22:00128587 RIV/00216208:11310/22:10479986
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S266616672200079X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S266616672200079X?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.xpro.2022.101199" target="_blank" >10.1016/j.xpro.2022.101199</a>

Result language
angličtina
Original language name
A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines
Original language description
Primary cilia are hair-like sensory organelles protruding from the surface of most human cells. As cilia are dynamic, several aspects of their biology can only be revealed by real-time analysis in living cells. Here we describe the generation of primary cilia reporter cell lines. Furthermore, we provide a detailed protocol of how to use the reporter cell lines for live-cell imaging microscopy analysis of primary cilia to study their growth as well as intraciliary transport.nnFor complete details on the use and execution of this protocol, please refer to Bernatik et al. (2020) and Pejskova et al. (2020).
Czech name
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Czech description
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Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
10601 - Cell biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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