Protocol for the induction of innate immune memory in human smooth muscle cells and endothelial cells in vitro
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F24%3A00585190" target="_blank" >RIV/68378050:_____/24:00585190 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11120/24:43926313
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S2666166723007438?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2666166723007438?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.xpro.2023.102776" target="_blank" >10.1016/j.xpro.2023.102776</a>
Alternative languages
Result language
angličtina
Original language name
Protocol for the induction of innate immune memory in human smooth muscle cells and endothelial cells in vitro
Original language description
Non-immune cells, like innate immune cells, can develop a memory-like phenotype in response to priming with microbial compounds or certain metabolites, which enables an enhanced response to a secondary unspecific stimulus. This paper describes a step-by-step protocol for the induction and analysis of trained immunity in human endothelial and smooth muscle cells. We then describe steps for cell culture with cryopreserved vascular cells, subcultivation, and induction of trained immunity. We then provide detailed procedures for downstream analysis using ELISA and qPCR.For complete details on the use and execution of this protocol, please refer to Sohrabi et al. (2020)1 and Shcnack et al.2
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
STAR protocols
ISSN
2666-1667
e-ISSN
2666-1667
Volume of the periodical
5
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
18
Pages from-to
102776
UT code for WoS article
001141119800001
EID of the result in the Scopus database
2-s2.0-85180279535