Magnesium effect on premelting transitions in nucleic acids: DNA duplex and RNA hairpin models

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F11%3A10108119" target="_blank" >RIV/00216208:11320/11:10108119 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0022286010009786" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0022286010009786</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.molstruc.2010.12.025" target="_blank" >10.1016/j.molstruc.2010.12.025</a>

Result language
angličtina
Original language name
Magnesium effect on premelting transitions in nucleic acids: DNA duplex and RNA hairpin models
Original language description
The effect of magnesium ions on the conformational changes in the temperature region below the melting transition (premelting transitions) of a DNA duplex and an RNA hairpin was studied by difference Raman spectroscopy. The chosen model systems were thepolynucleotide poly(dA)-poly(dT) duplex and the apical hairpin of the trans-activation response (TAR) element of HIV-1. Magnesium effect was displayed by differences of Raman spectra measured at various temperatures with and without magnesium ions. Our results have revealed that magnesium ions influence measurably the premelting transitions in both cases; the extent of the effect and its character is though quite different. In the case of the B'- B premelting transition of the polynucleotide poly(dA)-poly(dT) duplex, magnesium binds to the minor groove of the B but not of the B0 form and acts again the rearrangement in the vicinity of the thymidine keto-groups. On the other hand, the magnesium effect on the TAR hairpin premelting consis
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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