Morphological evidence of collateral sprouting of intact afferent and motor axons of the rat ulnar nerve by one type of tracer molecule

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F04%3A00010569" target="_blank" >RIV/00216224:14110/04:00010569 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Morphological evidence of collateral sprouting of intact afferent and motor axons of the rat ulnar nerve by one type of tracer molecule

Original language description

In our experimental paradigm we sutured distal stump of transected musculocutaneous nerve to an intact ulnar nerve of the rat by the end-to-side fashion. We demonstrated the formation of collateral sprouts from intact afferent and motor axons by application of one type of molecule conjugated by two different fluorophores (Fluoro-Ruby and Fluoro-Emerald). Fluoro-Ruby and Fluoro-Emerald were applied distal to end-to-side suture into fresh cut ends of the ulnar and musculocutaneous nerves, respectively. Formation of collateral sprouts was evidenced by findings of mixed (orange) fluorescence labeling of spinal motoneurons and dorsal root ganglia neurons. Colocalization of two different tracers retrogradely transported to the neurons was verified by green and red profiles analyzed by means of image analysis system. Our results unequivocally demonstrate that a nerve stump attached to an intact nerve can induce collateral sprouting of both afferent and motor axons.

Czech name

Morfogický důkaz tvorby kolaterál intaktních aferentních a motorických axonů v nervus ulnaris potkana pomocí jednoho typu transportované molekuly.

Czech description

K experimntům byl použit vlastní model end-to-side anastomosy, ve kterém byl distální pahýl přerušeného n. musculocutaneus našit do perineuriálního okna v n. ulnaris laboratorního potkana.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EA - Morphology and cytology

OECD FORD branch

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Project

<a href="/en/project/NF6658" target="_blank" >NF6658: Alternative approaches in brachial plexus reconstruction and experimental evaluation of various neurotrophic factors action on direct and collateral reinnervation</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Annals of Anatomy

ISSN

0940-9602

e-ISSN

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Volume of the periodical

186

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

4

Pages from-to

231-234

UT code for WoS article

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EID of the result in the Scopus database

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