Changes of immunofluorescence staining for ECM molecules in the rat dorsal root ganglia following chronic compression of the peripheral nerve

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F04%3A00010570" target="_blank" >RIV/00216224:14110/04:00010570 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Changes of immunofluorescence staining for ECM molecules in the rat dorsal root ganglia following chronic compression of the peripheral nerve

Original language description

The units of neuronal body-satellite cells in the dorsal root ganglia (DRG) are surrounded by the basal lamina and extracellular matrix (ECM) produced by the satellite cells. The neurons shift from normal maintenance and neurotransmission toward survivaland regeneration after axonal injury. However, scarce information is available on reaction of satellite cells following axotomy. The present experiments demonstrate changes of immunofluorescence staining for laminin-1, chondroitin sulfate proteoglycan (CSP), tenascin (TEN), thrombospondin (THS) and fibronectin (FN) in the DRG after intercostal nerve ligation. Twenty adult rats were deeply anesthetized and left intercostal nerves (8-12) were ligated by 6-0 Mersilk thread. Animals were allowed to survivefor 1 or 2 weeks. The corresponding DRG (Th8-12) of both sides were removed and immersed in Zamboni fixation. Intact DRG (Th8-12) were removed and fixed from other 4 rats. Longitudinal cryostat sections (12 ľm) through ipsilateral, contr

Czech name

Změny imunofluorescenčního barvení molekul ECM ve spinálních gangliích potkana po chronické kompresi periferního nervu.

Czech description

Funkční a morfologické jednotky neuron-satelitní buňky lokalizované ve spinálních gangliích jsou obklopeny bazální laminou a extracelulární matrix produkovanou satelitními buňkami. Ačkoliv jsou reakce satelitních buněk faktorem, který pravděpodobně ovlivňuje proces regenerace neuronu po poškození neuronu, je informací o těchto reakcích stále nedostatek. Práce se zabývá změnami imunofluorescenčního barvení pro laminin-1, chondroitin sulfát, tenascin, trombospondin a fibronektin ve spinálních gangliích potkana po ligatuře mezižeberních nervů.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EA - Morphology and cytology

OECD FORD branch

—

Project

<a href="/en/project/GA309%2F03%2F1199" target="_blank" >GA309/03/1199: Changes of the satellite glial cells and their extracellular matrix after axotomy - conditions for the sympathetic sprouts in the spinal ganglia?</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Molecular Histology

ISSN

1567-2379

e-ISSN

—

Volume of the periodical

35

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

1

Pages from-to

178-178

UT code for WoS article

—

EID of the result in the Scopus database

—