Intra- and Extraneuronal Changes of Immunofluorescence Staining for TNF-a and TNFR1 in the Dorsal Root Ganglia of Rat Peripheral Neuropathic Pain Models

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F06%3A00015796" target="_blank" >RIV/00216224:14110/06:00015796 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Intra- and Extraneuronal Changes of Immunofluorescence Staining for TNF-a and TNFR1 in the Dorsal Root Ganglia of Rat Peripheral Neuropathic Pain Models

Original language description

1. Several lines of evidence suggest that cytokines and their receptors are initiators of changes in the activity of dorsal root ganglia (DRG) neurons, but their cellular distribution is still very limited or controversial. Therefore, the goal of presentstudy was to investigate immunohistochemical distribution of TNF-a and TNF receptor-1 (TNFR1) proteins in the rat DRG following three types of nerve injury. 2. The unilateral sciatic and spinal nerve ligation as well as the sciatic nerve transection were used to induce changes in the distribution of TNF-a and TNFR1 proteins. The TNF-a and TNFR1 immunofluorescence was assessed in the L4-L5 DRG affected by nerve injury for 1 and 2 weeks, and compared with the contralateral ones and those removed from naive or sham-operated rats. A part of the sections was incubated for simultaneous immunostaining for TNF-a and ED-1. The immunofluorescence brightness was measured by image analysis system (LUCIA-G v4.21) to quantify immunostaining for TNF-

Czech name

Intra- a Extraneuronální změny v imunofluorescenci TNF-a a TNFR1 ve spinálních gangliích potkana v modelech periferní neuropatické bolesti

Czech description

Kvalitativní a kvantitativní imunohistochemická analýza proteinů TNF-a a jeho receptoru TNFR1 v ipsi- a kontralaterálních spinálních gangliích po různém poškození sedacího nervu (modelu neuropatické bolesti).

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FH - Neurology, neuro-surgery, nuero-sciences

OECD FORD branch

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Project

<a href="/en/project/GA309%2F03%2F1199" target="_blank" >GA309/03/1199: Changes of the satellite glial cells and their extracellular matrix after axotomy - conditions for the sympathetic sprouts in the spinal ganglia?</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cellular and Molecular Neurobiology

ISSN

1573-6830

e-ISSN

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Volume of the periodical

26

Issue of the periodical within the volume

4

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

13

Pages from-to

365-378

UT code for WoS article

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EID of the result in the Scopus database

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