Label-free voltammetric detection of single nucleotide mismatches recognized by MutS protein

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F07%3A00022108" target="_blank" >RIV/00216224:14110/07:00022108 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/07:00107888

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Label-free voltammetric detection of single nucleotide mismatches recognized by MutS protein

Original language description

MutS, a protein involved in DNA mismatch repair, recognizes mispaired and unpaired bases in duplex DNA. We have previously used MutS in an electrochemical double-surface technique (DST) for in-vitro detection of point mutations in DNA. The DST involved binding of unlabeled MutS to DNA heteroduplexes at the surface of magnetic beads followed by a highly sensitive electrochemical determination of the protein by measurement of a catalytic protein signal (peak H) at mercury electrodes. Detection of MutS using a peak resulting from oxidation of tyrosine and tryptophan residues of the protein at a carbon-paste electrode (CPE) was also possible but was approximately three orders of magnitude less sensitive. In this work we present an optimized technique for ex-situ voltammetric determination of MutS at a CPE. Choice of optimum experimental conditions (pH of supporting electrolyte, square-wave voltammetry settings, etc.) resulted in substantial improvement of the sensitivity of the assay, enab

Czech name

Voltametrická detekce změn jednoho nukletidu pomoci MutS proteinu bez pouziti znacek

Czech description

Voltametrická detekce změn jednoho nukletidu pomoci MutS proteinu bez pouziti znacek

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/LC06035" target="_blank" >LC06035: Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ANALYTICAL AND BIOANALYTICAL CHEMISTRY

ISSN

1618-2642

e-ISSN

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Volume of the periodical

388

Issue of the periodical within the volume

1

Country of publishing house

DE - GERMANY

Number of pages

12

Pages from-to

259-270

UT code for WoS article

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EID of the result in the Scopus database

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