Effect of dibenzocyclooctadiene lignans on multidrug resistant promyelotic leukaemia cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F12%3A00057524" target="_blank" >RIV/00216224:14110/12:00057524 - isvavai.cz</a>
Result on the web
<a href="http://biomed.papers.upol.cz/incpdfs/pri-990000-0200_10_26.pdf" target="_blank" >http://biomed.papers.upol.cz/incpdfs/pri-990000-0200_10_26.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Effect of dibenzocyclooctadiene lignans on multidrug resistant promyelotic leukaemia cells
Original language description
In this study we used multidrug resistant promyelotic leukaemia cells overexpressing MDR1 (P-glycoprotein), the most common member of ABC transporters family (HL60/ MDR). The ability to overcome multidrug resistance was examined in the panel of dibenzo[a,c]cyclooctadiene lignans, schizandrin, gomisin A, gomisin N, angeloylgomisin H, tigloylgomisin P, deoxyschizandrin-dicarbaldehyde, wuweizisu C, and S(-)- and R(+)-enantiomers of deoxyschizandrin, y-schizandrin and gomisin J. The lignans were isolated from Schisandra chinensis seeds or prepared semisynthetically. We observed that resistant HL60/MDR was nearly hundred times more resistant to doxorubicin than the parental line HL60; although both cell lines were similarly sensitive to lignans treatment, indicating that the lignans are not exported from the resistant cells. Using doxorubicin accumulation assay we demonstrated that all lignans significantly enhanced the accumulation of doxorubicin in drug resistant cells.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
FH - Neurology, neuro-surgery, nuero-sciences
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA522%2F07%2F0995" target="_blank" >GA522/07/0995: Regulation of secondary metabolites biosynthesis in cell culture of Schisandra chinensis</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů