Intra- and Interlaboratory variability of paroxysmal nocturnal hemoglobinuria testing by flow cytometry following the 2012 practical guidelines for high-sensitivity paroxysmal nocturnal hemoglobinuria testing

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F13%3A00070875" target="_blank" >RIV/00216224:14110/13:00070875 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11140/13:10135325 RIV/65269705:_____/13:#0002212 RIV/00669806:_____/13:10135325 RIV/00023736:_____/13:00010654
Result on the web
<a href="http://dx.doi.org/10.1002/cyto.b.21075" target="_blank" >http://dx.doi.org/10.1002/cyto.b.21075</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cyto.b.21075" target="_blank" >10.1002/cyto.b.21075</a>

Result language
angličtina
Original language name
Intra- and Interlaboratory variability of paroxysmal nocturnal hemoglobinuria testing by flow cytometry following the 2012 practical guidelines for high-sensitivity paroxysmal nocturnal hemoglobinuria testing
Original language description
Sutherland et al. recently published the Practical Guidelines for high-sensitivity detection of paroxysmal nocturnal hemoglobinuria (PNH) clones by flow cytometry (FCM), containing concise protocols for PNH testing. METHODS: Using this approach, we studied the intra- and interlaboratory variability observed in a multicenter study in which fresh blood samples containing three clinically relevant PNH clone sizes within the granulocytic, monocytic, and red blood cell (RBC) populations were shipped to eachparticipating center. RESULTS: Coefficients of variation (CVs) for precision/reproducibility analysis ranged from 0.01%/0.02% to 0.48%/0.45% (big clone), from 0.69%/1.52% to 4.24%/5.80% (small-intermediate clone), from 1.47%/3.91% to 15.01% /17.83% (minor clone) for PNH white blood cells (WBCs) and from 0.24%/0.48% to 1.76%/1.83% (big clone), from 0.80%/1.14% to 2.39%/4.45% (small-intermediate clone), from 1.09%/3.36% to 10.54%/10.23% (minor clone) for PNH RBCs, respectively.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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