Structure and Dynamics of DNA Duplexes Containing a Cluster of Mutagenic 8-Oxoguanine and Abasic Site Lesions
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F14%3A00078633" target="_blank" >RIV/00216224:14110/14:00078633 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.jmb.2013.12.022" target="_blank" >http://dx.doi.org/10.1016/j.jmb.2013.12.022</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jmb.2013.12.022" target="_blank" >10.1016/j.jmb.2013.12.022</a>
Alternative languages
Result language
angličtina
Original language name
Structure and Dynamics of DNA Duplexes Containing a Cluster of Mutagenic 8-Oxoguanine and Abasic Site Lesions
Original language description
Clustered DNA damage sites are caused by ionizing radiation. They are much more difficult to repair than are isolated single lesions, and their biological outcomes in terms of mutagenesis and repair inhibition are strongly dependent on the type, relativeposition and orientation of the lesions present in the cluster. To determine whether these effects on repair mechanism could be due to local structural properties within DNA, we used H-1 NMR spectroscopy and restrained molecular dynamics simulation to elucidate the structures of three DNA duplexes containing bistranded clusters of lesions. Each DNA sequence contained an abasic site in the middle of one strand and differed by the relative position of the 8-oxoguanine, staggered on either the 3' or the 5' side of the complementary strand. Their repair by base excision repair protein Fpg was either complete or inhibited. All the studied damaged DNA duplexes adopt an overall B-form conformation and the damaged residues remain intrahelical.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Molecular Biology
ISSN
0022-2836
e-ISSN
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Volume of the periodical
426
Issue of the periodical within the volume
7
Country of publishing house
GB - UNITED KINGDOM
Number of pages
15
Pages from-to
1524-1538
UT code for WoS article
000334478000015
EID of the result in the Scopus database
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