The Effect of Voltage-Sensitive Dye di-4-ANEPPS on Heart Rate Variability in Langendorff-Perfused Isolated Rabbit Heart

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F15%3A00081552" target="_blank" >RIV/00216224:14110/15:00081552 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
The Effect of Voltage-Sensitive Dye di-4-ANEPPS on Heart Rate Variability in Langendorff-Perfused Isolated Rabbit Heart
Original language description
Optical mapping of heart electrical activity in Langendorff-perfused isolated hearts is based on voltagesensitive dyes, of which the most commonly used one is di-4-ANEPPS. Prolongation of action potential duration by di-4-ANEPPS was reported in isolatedcells; however, this phenomenon was not observed in the whole heart model. In order to search for other adverse phenomena of di- 4-ANEPPS use, its effect on heart rate variability (HRV) was investigated during staining and washout periods in five New Zealand White rabbit isolated hearts. Time domain, frequency domain and non-linear HRV parameters (21 in total) revealed that there are no significant changes between control, staining and washout periods. Voltage-sensitive dye di-4-ANEPPS does not affect HRV when applied into the isolated heart coronary system. It can be concluded that di-4-ANEPPS may be safely used in studies combining optical mapping and HRV analysis
Czech name
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Czech description
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Project
<a href="/en/project/GAP102%2F12%2F2034" target="_blank" >GAP102/12/2034: Analysis of Relationship between Electrical Activity and Blood Flow at the Heart Ventricles</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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