Non-DHFR-mediated effects of methotrexate in osteosarcoma cell lines: epigenetic alterations and enhanced cell differentiation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F16%3A00088886" target="_blank" >RIV/00216224:14110/16:00088886 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1186/s12935-016-0289-2" target="_blank" >http://dx.doi.org/10.1186/s12935-016-0289-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s12935-016-0289-2" target="_blank" >10.1186/s12935-016-0289-2</a>
Alternative languages
Result language
angličtina
Original language name
Non-DHFR-mediated effects of methotrexate in osteosarcoma cell lines: epigenetic alterations and enhanced cell differentiation
Original language description
Methotrexate is an important chemotherapeutic drug widely known as an inhibitor of dihydrofolate reductase (DHFR) which inhibits the reduction of folic acid. DHFR-mediated effects are apparently responsible for its primary antineoplastic action. However, other non-DHFR-mediated effects of methotrexate have been recently discovered, which might be very useful in the development of new strategies for the treatment of pediatric malignancies. The principal goal of this study was to analyze the possible impact of clinically achievable methotrexate levels on cell proliferation, mechanisms of epigenetic regulation (DNA methylation and histone acetylation), induced differentiation and the expression of differentiation-related genes in six osteosarcoma cell lines. Methotrexate significantly decreased the proliferation of Saos-2 cells exclusively, suggesting that this reference cell line was sensitive to the DHFR-mediated effects of methotrexate.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FG - Paediatrics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/NT14327" target="_blank" >NT14327: DHFR- and non-DHFR-mediated effects of methotrexate on cellular level: a study on cell lines derived from pediatric solid tumors</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cancer Cell International
ISSN
1475-2867
e-ISSN
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Volume of the periodical
16
Issue of the periodical within the volume
14
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
"nestránkováno"
UT code for WoS article
000371005700001
EID of the result in the Scopus database
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