Fruit peel polyphenols demonstrate substantial anti-tumour effects in the model of breast cancer

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F16%3A00124591" target="_blank" >RIV/00216224:14110/16:00124591 - isvavai.cz</a>

Alternative codes found

RIV/00159816:_____/16:00063275

Result on the web

<a href="https://link.springer.com/article/10.1007/s00394-015-0910-5" target="_blank" >https://link.springer.com/article/10.1007/s00394-015-0910-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00394-015-0910-5" target="_blank" >10.1007/s00394-015-0910-5</a>

Result language

angličtina

Original language name

Fruit peel polyphenols demonstrate substantial anti-tumour effects in the model of breast cancer

Original language description

Purpose Fruit and vegetable intake is inversely correlated with cancer; thus, it is proposed that an extract of phytochemicals as present in whole fruits, vegetables, or grains may have anti-carcinogenic properties. Thus, the anti-tumour effects of fruit peel polyphenols (Flavin7) in the chemoprevention of N-methyl-N-nitrosourea-induced mammary carcinogenesis in female rats were evaluated. Methods Lyophilized substance of Flavin7 (F7) was administered at two concentrations of 0.3 and 3 % through diet. The experiment was terminated 14 weeks after carcinogen administration, and mammary tumours were removed and prepared for histopathological and immunohistochemical analysis. In addition, using an in vitro cytotoxicity assay, apoptosis and proliferation after F7 treatment in human breast adenocarcinoma (MCF-7) cells were performed. Results High-dose F7 suppressed tumour frequency by 58 % (P &lt; 0.001), tumour incidence by 24 % (P &lt; 0.05), and lengthened latency by 8 days (P &gt; 0.05) in comparison with the control rats, whereas lower dose of F7 was less effective. Histopathological analysis of tumours showed significant decrease in the ratio of high-/low-grade carcinomas after high-dose F7 treatment. Immunohistochemical analysis of rat carcinoma cells in vivo found a significant increase in caspase-3 expression and significant decrease in Bcl-2, Ki67, and VEGFR-2 expression in the high-dose group. Both doses demonstrated significant positive effects on plasma lipid metabolism in rats. F7 significantly decreased survival of MCF-7 cells in vitro in MTT assay by dose- and time-dependent manner compared to control. F7 prevented cell cycle progression by significant enrichment in G1 cell populations. Incubation with F7 showed significant increase in the percentage of annexin V-/PI-positive MCF-7 cells and DNA fragmentation. Conclusions Our results reveal a substantial tumour-suppressive effect of F7 in the breast cancer model. We propose that the effects of phytochemicals present in this fruit extract are responsible for observed potent anti-cancer activities.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30212 - Surgery

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

EUROPEAN JOURNAL OF NUTRITION

ISSN

1436-6207

e-ISSN

1436-6215

Volume of the periodical

55

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

11

Pages from-to

955-965

UT code for WoS article

000374112900007

EID of the result in the Scopus database

2-s2.0-84928658549