Osteoarthritic process modifies expression response to NiTi alloy presence

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F18%3A00101160" target="_blank" >RIV/00216224:14110/18:00101160 - isvavai.cz</a>

Alternative codes found

RIV/68081723:_____/18:00496634

Result on the web

<a href="http://dx.doi.org/10.1007/s10856-018-6156-z" target="_blank" >http://dx.doi.org/10.1007/s10856-018-6156-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10856-018-6156-z" target="_blank" >10.1007/s10856-018-6156-z</a>

Result language

angličtina

Original language name

Osteoarthritic process modifies expression response to NiTi alloy presence

Original language description

Nickel-titanium alloy (nitinol, NiTi) is a biomaterial with unique thermal shape memory, superelasticity and high damping properties. Therefore NiTi has been used in medical applications. In this in vitro study, the effect of NiTi alloy (with two surface modifications - helium and hydrogen) on gene expression profile of selected interleukins (IL-1, IL-6 and IL-8) and matrix metalloproteinases (MMP-1 and MMP-2) in human physiological osteoblasts and human osteoarthritic osteoblasts was examined to respond to a question of the different behavior of bone tissue in the implantation of metallic materials in the presence of cells affected by the osteoarthritic process. The cells were cultivated in contact with NiTi and with or without LPS (bacterial lipolysaccharide). Changes in expression of target genes were calculated by 2(-Ct) method. An increased gene expression of IL-1 in osteoarthritic osteoblasts, with even higher expression in cells collected directly from the metal surface was observed. In case of physiological osteoblasts, the change in expression was detected after LPS treatment in cells surrounding the disc. Higher expression levels of IL-8 were observed in osteoarthritic osteoblasts after NiTi treatment in contact with alloy, and in physiological osteoblasts without relation to location in combination of NiTi and LPS. IL-6 was slightly increased in physiological osteoblastes after application of LPS. MMP-1 expression level was obviously significantly higher in osteoarthritic osteoblasts with differences regarding the metal surface and location. MMP-2 expression was decreased in both cell lines after LPS treatment. In conclusion, results of present study show that the NiTi alloy and the treatment by LPS, especially repeated doses of LPS, change the gene expression of selected ILs and MMPs in human osteoblast cell cultures. Some of the changes were depicted solely to osteoarthritic osteoblasts.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20601 - Medical engineering

Project

<a href="/en/project/GA15-16336S" target="_blank" >GA15-16336S: Interstitial impurities in NiTi-based shape memory alloys</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Materials Science: Materials in Medicine

ISSN

0957-4530

e-ISSN

1573-4838

Volume of the periodical

29

Issue of the periodical within the volume

9

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

12

Pages from-to

1-12

UT code for WoS article

000443368900001

EID of the result in the Scopus database

2-s2.0-85052628154