Phospho-Protein Arrays as Effective Tools for Screening Possible Targets for Kinase Inhibitors and Their Use in Precision Pediatric Oncology
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F19%3A00108599" target="_blank" >RIV/00216224:14110/19:00108599 - isvavai.cz</a>
Alternative codes found
RIV/65269705:_____/19:00071258
Result on the web
<a href="https://www.frontiersin.org/articles/10.3389/fonc.2019.00930/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fonc.2019.00930/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fonc.2019.00930" target="_blank" >10.3389/fonc.2019.00930</a>
Alternative languages
Result language
angličtina
Original language name
Phospho-Protein Arrays as Effective Tools for Screening Possible Targets for Kinase Inhibitors and Their Use in Precision Pediatric Oncology
Original language description
The specific targeting of signal transduction by low-molecular-weight inhibitors or monoclonal antibodies represents a very promising personalized treatment strategy in pediatric oncology. In this study, we present the successful and clinically relevant use of commercially available phospho-protein arrays for analyses of the phosphorylation profiles of a broad spectrum of receptor tyrosine kinases and their downstream signaling proteins in tumor tissue samples. Although these arrays were made for research purposes on human biological samples, they have already been used by several authors to profile various tumor types. Our study performed a systematic analysis of the advantages and pitfalls of the use of this method for personalized clinical medicine. In certain clinical cases and their series, we demonstrated the important aspects of data processing and evaluation, the use of phospho-protein arrays for single sample and serial sample analyses, and the validation of obtained results by immunohistochemistry, as well as the possibilities of this method for the hierarchical clustering of pediatric solid tumors. Our results clearly show that phospho-protein arrays are apparently useful for the clinical consideration of druggable molecular targets within a specific tumor. Thus, their potential validation for diagnostic purposes may substantially improve the personalized approach in the treatment of relapsed or refractory solid tumors.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30204 - Oncology
Result continuities
Project
<a href="/en/project/NV16-34083A" target="_blank" >NV16-34083A: Receptor tyrosin kinases and downstream signaling pathways as possible therapeutic targets in pediatric refractory solid tumors</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Oncology
ISSN
2234-943X
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
SEP 20
Country of publishing house
CH - SWITZERLAND
Number of pages
11
Pages from-to
1-11
UT code for WoS article
000487240200001
EID of the result in the Scopus database
2-s2.0-85075835727