OP12 - IN VITRO “GLICO” MODEL FOR STUDYING BRAIN CANCER PATHOGENESIS

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F24%3A00137761" target="_blank" >RIV/00216224:14110/24:00137761 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

OP12 - IN VITRO “GLICO” MODEL FOR STUDYING BRAIN CANCER PATHOGENESIS

Original language description

Advancements in preclinical models are pivotal for enhancing our understanding of complex diseases like glioblastoma. Our research team has developed a sophisticated in vitro model utilizing the co-culture of glioblastoma tumoroids and cerebral organoids (termed “GLICO”; Fig.1A), aiming to replicate the tumor microenvironment and investigate glioblastoma cell behavior. Leveraging extensive expertise in stem cell biology and neurodifferentiation, we have differentiated cerebral organoids from healthy induced pluripotent stem cells. We then integrated mature brain organoids with fluorescently labeled glioblastoma cell line U-87 (Fig.1B). This model now facilitates a detailed study of glioblastoma growth dynamics, and migration patterns (Fig.1C), and also allows us to specifically analyze glioblastoma cells after sorting. The pivotal findings, documented in our recent publication [1], underscore the model’s efficacy in mirroring the complex biological processes of glioblastoma, highlighted by significant alterations in glioblastoma cell fate upon interaction with the brain organoids. Efforts are underway to optimize this system for the co-culture of organoids with patients-derived tumoroids to provide a better gateway to discovering potential therapeutic targets and intervention strategies.

Czech name

—

Czech description

—

Type

O - Miscellaneous

CEP classification

—

OECD FORD branch

10601 - Cell biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů