Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F23%3A00571228" target="_blank" >RIV/68378050:_____/23:00571228 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/23:00571228 RIV/00216224:14110/23:00130401 RIV/00159816:_____/23:00079646
Result on the web
<a href="https://febs.onlinelibrary.wiley.com/doi/full/10.1002/1878-0261.13389" target="_blank" >https://febs.onlinelibrary.wiley.com/doi/full/10.1002/1878-0261.13389</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/1878-0261.13389" target="_blank" >10.1002/1878-0261.13389</a>

Result language
angličtina
Original language name
Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration
Original language description
It is currently challenging to adequately model the growth and migration of glioblastoma using two-dimensional (2D) in vitro culture systems as they quickly lose the original, patient-specific identity and heterogeneity. However, with the advent of three-dimensional (3D) cell cultures and human-induced pluripotent stem cell (iPSC)-derived cerebral organoids (COs), studies demonstrate that the glioblastoma-CO (GLICO) coculture model helps to preserve the phenotype of the patient-specific tissue. Here, we aimed to set up such a model using mature COs and develop a pipeline for subsequent analysis of cocultured glioblastoma. Our data demonstrate that the growth and migration of the glioblastoma cell line within the mature COs are significantly increased in the presence of extracellular matrix proteins, shortening the time needed for glioblastoma to initiate migration. We also describe in detail the method for the visualization and quantification of these migrating cells within the GLICO model. Lastly, we show that this coculture model (and the human brain-like microenvironment) can significantly transform the gene expression profile of the established U87 glioblastoma cell line into proneural and classical glioblastoma cell types.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30204 - Oncology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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