Chromatographic behaviour of linear DNA molecules of defined size

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F00%3A00003594" target="_blank" >RIV/00216224:14310/00:00003594 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Chromatographic behaviour of linear DNA molecules of defined size
Original language description
The aim of our work was to study the chromatographic behaviour of lambda DNA (48.5 kbp) and lambda/HindIII DNA fragments (23.1, 9.4, 6.6, 4.4, 2.3, 2.0, 0.6, 0.1 kbp) using sorbents based on HEMA-co-EDMA. The ion-exchange chromatography (IEC) and gel permeation chromatography (GPC) were used for DNA fragment separations. The resolution of larger lambda/HindIII fragments (23.1 and 9.4 kbp) was insufficient using IEC. In the case of GPC, DNA fragments smaller than 9.4 kbp were not separated from each other and were eluted in the void volume. The order of the separation of relatively large DNA fragments (larger then 9.4 kbp) and lambda DNA molecules was reverse to the order expected for GPC. This is in agreement with the principle of slalom chromatography.
Czech name
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Czech description
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Project
<a href="/en/project/GA203%2F98%2F1231" target="_blank" >GA203/98/1231: Oriented immobilization of enzymes and antibodies using biospecific interactions</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2000
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
20th International Symposium on the Separation and Analysis of Proteins, Peptides and Polynucleotides
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Number of pages
1
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Publisher name
University of Ljubljana
Place of publication
Ljubljana
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UT code for WoS article
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