Chromatographic purification and separation of plasmid and lambda phage DNAs

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F02%3A00006665" target="_blank" >RIV/00216224:14310/02:00006665 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Chromatographic purification and separation of plasmid and lambda phage DNAs
Original language description
Plasmid pBR322 DNA was purified by gel permeation chromatography. Recovered plasmid DNA was free of RNA and retained its biological activity. The biological activity of purified plasmid DNA was tested by electrotransformation into bacterial cells. DNA fragments of lambda DNA and lambda DNA were separated on column for gel permeation chromatography. Small fragments were eluted in void volume; large fragments and lambda DNA were separated in reversed order than expected according to GPC and according to the slalom chromatography mechanisms.
Czech name
Chromatographic purification and separation of plasmid and lambda phage DNAs
Czech description
Plasmid pBR322 DNA was purified by gel permeation chromatography. Recovered plasmid DNA was free of RNA and retained its biological activity. The biological activity of purified plasmid DNA was tested by electrotransformation into bacterial cells. DNA fragments of lambda DNA and lambda DNA were separated on column for gel permeation chromatography. Small fragments were eluted in void volume; large fragments and lambda DNA were separated in reversed order than expected according to GPC and according to the slalom chromatography mechanisms.

Project
<a href="/en/project/GA203%2F00%2F1339" target="_blank" >GA203/00/1339: Dispersion polymerisation of hydrophilic monomers for the preparation of magnetic carriers used in biotechnological application</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2002
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
22nd International Symposium on the Separation of Proteins, Peptides and Polynucleotides
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
53-53
Publisher name
Dechema
Place of publication
Heidelberg, Germany
Event location
November, 10-13, 2002, Heidelberg, Germany
Event date
Jan 1, 2002
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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