Chromatographic separation of lambda DNas
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F99%3A00004113" target="_blank" >RIV/00216224:14310/99:00004113 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Chromatographic separation of lambda DNas
Original language description
The separation of DNA fragments are of increasing interest of molecular biology, gene technology and applied sciences. The most widely used methods, the polyacrylamide and agarose gel electrophoresis, have been limited to comparatively small molecules. Moreover the fragments are contamined by soluble impurities from gels, which are known to be inhibitors of DNA processing enzymes. The separation of DNA fragments larger than 20 kbp, which are important as inserts in recombinant DNA research, has been studied. An alternative method based on gel permeation chromatography (GPC) has been adopted for this purpose. In our work GPC on HEMA based sorbents (copolymer of 2-hydroxyethyl methacrylate and ethylene dimethacrylate) was used for separation of wild typelambda DNA molecules (48.5 kbp), their concatemers and mixture of lambda DNA fragments. Three peaks representing the 9.4, 23.1 and 48.5 kbp were eluted in the order of the slalom mode. The separation of lambda DNA concatemers was not ach
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA203%2F98%2F1231" target="_blank" >GA203/98/1231: Oriented immobilization of enzymes and antibodies using biospecific interactions</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
1999
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
13th Bratislava International Conference on Polymers. Separation and Characterization of Macromolecules
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
141
Publisher name
Polymer Institute of the Slovak Academy of Sciences
Place of publication
Bratislava
Event location
Bratislava
Event date
Jan 1, 1999
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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