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Application of PCR, rep-PCR and RAPD techniques for typing of Lactococcus lactis strains

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00014203" target="_blank" >RIV/00216224:14310/05:00014203 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Application of PCR, rep-PCR and RAPD techniques for typing of Lactococcus lactis strains

  • Original language description

    A collection of 34 lactococcal strains were characterized using PCR for acmA gene, and for 16S rDNA gene, and DNA fingerprinting methods for randomly amplified polymorphic DNA (RAPD) and repetitive extragenic palindromes-PCR (rep-PCR). PCR experiments enabled the genotypic identification of L. lactis strains by RAPD. Rep-PCR did not distinguish between L. lactis subspecies. In some cases, phenotypic classification of L. lactis subspecies did not correlate with genotypic characterizations.

  • Czech name

    Využití technik PCR, rep-PCR a RAPD pro typizaci kmenů Lactococcus lactis

  • Czech description

    Soubor 34 kmenů laktokoků byl charakterizován pomocí PCR pro acmA a 16S rDNA geny,RAPD a rep-PCR. Genotypová identifikace kmenů L. lactisbyla umožněna použitím RAPD. Rep-PCR neumožnila rozlišení podruhů L. lactis. V některých případech fenotypická klasifikace podruhů L. lactis nekorelovala s genotypovou charakterizací.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2005

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Folia Microbiologica

  • ISSN

    0015/5632

  • e-ISSN

  • Volume of the periodical

    50

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    5

  • Pages from-to

    150-154

  • UT code for WoS article

  • EID of the result in the Scopus database