NEW MODEL FOR EVALUATION OF ENDOCRINE DISRUPTION OF STEROIDOGENESIS BASED ON ADRENOCORTICAL CELL LINE H295R

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00014605" target="_blank" >RIV/00216224:14310/05:00014605 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

NEW MODEL FOR EVALUATION OF ENDOCRINE DISRUPTION OF STEROIDOGENESIS BASED ON ADRENOCORTICAL CELL LINE H295R

Original language description

Endocrine disrupting mechanisms of xenobiotics that are released to the environment in large quantities has been very important subject of scientific studies in last years. Main concern was focused on comprehending the process of interaction of xenobiotics with estrogenic receptor which was perceived as the most considerable point of action of endocrine disruptors. As it was shown later, other parts of the steroid signaling pathway are no less important for endocrine disruptors mode of action. Steroidogenesis as a very complex process represents one of them. We used human adrenocorticoid carcinoma cell line H295R which retains ability to synthesize complete set of steroids to develop a model suitable for assessment of the effects of xenobiotics on production of main steroids. Cells were exposed to forskolin (activator of steroidogenesis) and/or tested compound. Levels of main steroids (17-estradiol, testosterone, cortisol, aldosterone, 17-OH-progesterone) were assessed directly in expo

Czech name

NEW MODEL FOR EVALUATION OF ENDOCRINE DISRUPTION OF STEROIDOGENESIS BASED ON ADRENOCORTICAL CELL LINE H295R

Czech description

Endocrine disrupting mechanisms of xenobiotics that are released to the environment in large quantities has been very important subject of scientific studies in last years. Main concern was focused on comprehending the process of interaction of xenobiotics with estrogenic receptor which was perceived as the most considerable point of action of endocrine disruptors. As it was shown later, other parts of the steroid signaling pathway are no less important for endocrine disruptors mode of action. Steroidogenesis as a very complex process represents one of them. We used human adrenocorticoid carcinoma cell line H295R which retains ability to synthesize complete set of steroids to develop a model suitable for assessment of the effects of xenobiotics on production of main steroids. Cells were exposed to forskolin (activator of steroidogenesis) and/or tested compound. Levels of main steroids (17-estradiol, testosterone, cortisol, aldosterone, 17-OH-progesterone) were assessed directly in expo

Type

D - Article in proceedings

CEP classification

DN - Environmental impact on health

OECD FORD branch

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Project

<a href="/en/project/GP525%2F05%2FP160" target="_blank" >GP525/05/P160: In vitro models for study of endocrine disruption - effects of traditional and new types of persistent organic pollutants</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Chemicals, human and environment

ISBN

9949-13-297-5

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

71

Publisher name

ETS

Place of publication

Tallin

Event location

Toila, Estonsko

Event date

Oct 20, 2005

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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